Table of contentsGenome variation discovery and genotyping: approaches for 2004 Variation discovery Slide 3 PCR (Kary Mullis 1983) Genotyping Methods Hybridization assay SNP genotyping by hybridization Reverse dot blot Enhanced hybridization methods TaqMan assay for SNP Genotyping Slide 11 Slide 12 PowerPoint Presentation GeneChip® Mapping 10K Array (Affymetrix) Allele specific PCR for SNP genotyping Read-out methods for allele specific PCR ‘Kinetic’ PCR for allele-specific PCR discrimination Slide 18 Minisequencing for SNP genotyping SNP SNaPshot Assay Pyrosequencing Sequenom MassEXTEND 3-plex Spectrum Scatterplot Positives/negatives of Sequenom genotyping by mass spec Fluorescence polarization: PE Acycloprime FP Fluorescence polarization Slide 28 Minisequencing for SNP genotyping extensions and improvements Illumina BeadArray Slide 31 Slide 32 Ligation chain reaction (LCR) Ligation methods AB SNPlexTM Genotyping a new ligation-based method AB SNPlexTM Genotyping ParAllele Molecular Inversion Probes Slide 38 Slide 39 Cleavage methods Third Wave Invader assay Genome variation discovery and genotyping: technologies and applications Genotyping Technologies for 2004-> |
Author: David Kwiatkowski Homepage: https://innateimmunity.net/ |